FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004172753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004172753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52099207
Sequences flagged as poor quality0
Sequence length25-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG30850.021.63701645
TAATGCG31550.021.10407434
CCCGTAT95850.016.0618021
AATGCGC43350.015.77730235
TATCGCG62450.015.764400522
GTATCGA90800.015.29667954
AGGCGAC99750.015.16327410
CGTATCG112200.013.8013413
TATCGAA105300.013.7452315
CCGTATC120600.013.4736972
ATATCGC74850.013.332499521
GGCGACA115550.013.18713811
GCGACAG118800.012.99663912
TCGCGGC79950.012.93292724
GATCGGA53500.012.6867081
ACACGTC55150.012.67488513
ATCGAAG161500.012.4132896
GCGCTAT6950.012.2883791
ACGTCTG56300.012.25664615
CGACAGC125650.012.19975313
Produced by FastQC (version 0.11.5)