FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004173989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004173989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386664557
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG669450.017.2812523
GTATCGA695650.016.8830574
CCCGTAT798650.016.5058921
CGACCGT253800.013.283511
ATCGAAG1375050.010.9860976
TATCGAA1157400.010.6923255
CGGTAAT364200.010.2604761
CCGTATC1210850.010.2484552
CGGGGAT955550.09.703761
CGACCTA261500.09.4113394
CCGGGAT977750.09.3752211
TCGAAGG1669300.09.0203737
CGGGGTT1268900.08.98651
CCCGGAT1178350.08.917921
CTCGGAT1066950.08.8213121
GCGAACG219800.08.7975373
ATCGCGC715750.08.6339466-67
CGGCGAA446000.08.5619381
TAAGGCG796350.08.1720498
CCCGGTT686900.07.98874571
Produced by FastQC (version 0.11.5)