FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004173994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004173994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380851390
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG546450.018.599183
GTATCGA588300.017.6966294
CCCGTAT693400.017.0360051
CGACCGT237450.016.118251
CCGTCAT1172400.015.4224261
CCGTATC734200.015.1570542
GACGCTA587700.013.9729514
TACTCTA1366800.012.7462339
CGGTAAT401150.012.6612431
ACGCTAC664700.012.5295095
GCGCTAA874000.010.95838842-43
CTTGGAT2681400.010.7551571
TAAGGCG1432950.010.606818
CGTCATC1660150.010.39825252
CGGGGAT920350.010.2711921
TAACCGG966800.010.23296454
CTCGGAT883000.09.7225351
TTAAGGC1700050.09.6190367
GGGTAAT1623800.09.3866051
CGGGGTT1184950.09.1675931
Produced by FastQC (version 0.11.5)