FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004174010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386701445
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1093050.027.1485344-45
CCGGCTA1399700.021.60417232-33
TATGCCG1056600.019.16694646-47
GTATGCC1568500.019.102446-47
CGGCTAT1186250.017.69635232-33
CTCGTAT1171900.017.22030842-43
TCTCGTA1646550.017.13822742-43
CGTATGC1224750.016.66726144-45
GCCGTCT2091500.014.46300950-51
GTCACCG2145000.014.29237628-29
CACCGGC2215750.013.98070730-31
TATGATC1441300.013.37869636-37
ATGCCGT2352950.013.005342548-49
ACCGGCT1620500.012.9610130-31
ACGTCTG2425550.012.77230714-15
GTATCGA596650.012.6248264
CGTATCG695350.012.4452283
CCCGTAT775850.012.1327151
CTATGAT2401400.012.059504536-37
GATCTCG2480000.011.73290840-41
Produced by FastQC (version 0.11.5)