FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004174012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386562797
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA402000.020.26815434-35
CCCGTAT632350.016.4788991
CGTATCG502750.015.8998143
CCGCGAA553750.015.76025134-35
TATGCCG542050.015.30485946-47
CCGTATC694200.014.0505742
GTATCGA578850.013.51302054
TCGTATG665700.013.299849544-45
CTCGTAT647200.012.94947542-43
TCCGCGA673350.012.47498232-33
CGTATGC690000.012.25666544-45
CCCGTCT1683350.011.5007511
GCGAAAT770250.011.364243536-37
CGAAATC774800.010.47302736-37
TATCGAA780250.010.3963035
CTCGGAT996350.010.2124641
GTCCGAT433750.09.8335651
CTCGTTT789800.09.7963821
TCAATCG824550.09.1808328
CGGGTAT357300.09.1651771
Produced by FastQC (version 0.11.5)