FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004174013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386562797
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA450950.017.84162956-57
GCCGTAT538950.015.963757558-59
CGTATCA598600.014.45743360-61
CCGTATC671750.012.503818558-59
TCGCCGT884450.010.05268556-57
TCGACCG408050.09.2443687
GTCGCCG936500.09.06781754-55
TCTCGGT971250.08.86131146-47
TGGTCGC990050.08.66105452-53
GTAGATC1062250.08.5599942-43
GATTAGT995850.08.53807334-35
GGTCGCC1070200.08.48749454-55
CGACCGT283800.08.2425071
TAGTGTA1126650.08.17180238-39
CGGTAAT319700.07.88321261
GTAATCG299150.07.6875933
TACCGTA593200.07.6398159
TCGCATA229600.07.57319747
GACGCTA727000.07.27924549
GTAGGTC695650.07.2527692
Produced by FastQC (version 0.11.5)