FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004174015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409181047
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA576550.025.38059456-57
CGTATCA638850.024.08712260-61
GCCGTAT730450.021.1634758-59
CCGTATC706450.020.82730758-59
GTACGTC878800.017.6713230-31
TCACCCG1608500.016.3438133
TACGTCC958250.015.45521430-31
CTCGATT1040750.015.20972948-49
ACCCGGC1749250.015.0689875
TCTCGGT1016650.014.71946246-47
GTGTACG1070350.014.63259428-29
CACCCGG1846600.014.3393344
TAGCATA1129850.014.2646739
TAGATCT1070950.014.16083942-43
TGTACGT1041850.014.14831728-29
ATTCCGT1136200.013.88738752-53
GTATCAT1122850.013.65372360-61
TTCTCGA1182850.013.5527746-47
GGTGTAG1185100.012.78740138-39
GATCTCG1200250.012.569242544-45
Produced by FastQC (version 0.11.5)