FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004174016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406106610
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGA652400.021.69376434-35
TATGCCG662850.021.34513946-47
TCGTATG830400.019.64104344-45
CTCGTAT760050.018.59698142-43
CCCGTAT823400.017.5023121
GCGATAG931050.017.33081834-35
CGTATCG802400.016.6261163
CGTATGC873300.016.41645244-45
CCGTATC940100.015.7771262
GTATCGA826450.015.31461054
TCTCGTA1116900.013.35901542-43
GTATGCC1263800.013.17956746-47
AGCGATA1133600.012.80980432-33
TATCGAA1042200.012.3440325
TCAATCG1220550.011.5135748
CAATCGG1211800.011.2322299
ATCGAAG1398700.011.0550046
ACGTCTG1517500.011.0517614-15
GCCGTCT1566050.010.80129950-51
ACACGTC1561250.010.6978212-13
Produced by FastQC (version 0.11.5)