FastQCFastQC Report
Tue 4 Apr 2023
EGAF00004174228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79128901
Sequences flagged as poor quality0
Sequence length80
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3586390.4532338949077531TruSeq Adapter, Index 5 (100% over 50bp)
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA1494560.18887662802242128No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1434240.1812536231231115No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1417690.17916209906668612No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG902350.11403545210365046No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA855070.10806039123429757No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT850000.10741966452939868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)