FastQCFastQC Report
Mon 6 Mar 2023
EGAF00004174246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127415319
Sequences flagged as poor quality0
Sequence length80
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA4833270.3793319388856217No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT4141460.3250362697753792TruSeq Adapter, Index 19 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2118250.16624767073729965No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1783250.13995569873352512No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1374340.10786301135423128No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1293910.10155058356836984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)