FastQCFastQC Report
Thu 14 Sep 2023
EGAF00004174257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66723381
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAAA2685360.4024616198630582Illumina Single End PCR Primer 1 (100% over 56bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA2274050.3408175613882636Illumina Single End PCR Primer 1 (100% over 57bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGG836530.1253728434414917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)