FastQCFastQC Report
Tue 8 Aug 2023
EGAF00004174262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73770319
Sequences flagged as poor quality0
Sequence length80
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3982700.539878375746213TruSeq Adapter, Index 3 (100% over 50bp)
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA1626550.2204884053707291No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1253210.1698799757121831No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1141980.15480209594864297No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT1019840.13824530160971651No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG926530.1255965830919072No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC754460.10227148400971399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)