FastQCFastQC Report
Fri 24 Mar 2023
EGAF00004174270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88161069
Sequences flagged as poor quality0
Sequence length80
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2386420.27068864149095107TruSeq Adapter, Index 20 (97% over 44bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2133690.2420217930887385No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1839160.20861362286793503No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1560890.1770498041488131No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1094020.12409332287021156No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1024920.11625539613182323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)