FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004174308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760253
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA804702.9153124731682203TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA107090.38797168230593354TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG94680.34301203549094955TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)