FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5110442
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1184792.3183708962942933No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG173960.34040108468112934No Hit
GGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTG154530.3023808899504191No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA114140.2233466302914699No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG102210.200002269862372No Hit
CCATGGGTCTGGATTTCCAGGAAAGCGGAGACCTCGAGGTGCAGGACTGTCGGGGCGAGGTGG79370.15530946246919544No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT74900.1465626652254345No Hit
CCTACATCAAATCCCCAGCACAGGGTCCCATCCATCTCCCCATTACCTTT53890.10545076140185135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)