FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6026504
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2598974.312566622373436No Hit
AGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTATGCCGTCT232560.3858953715122399TruSeq Adapter, Index 5 (96% over 30bp)
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA212410.3524597345326577No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC108320.1797393646465679No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC96020.1593295217260289No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG87540.14525834546861663No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA85110.14122615698919308No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTATGC80300.13324474687148635TruSeq Adapter, Index 5 (97% over 35bp)
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG78500.1302579405904319No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT67830.11255281669106999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)