FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6026504
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2439214.047470971561622No Hit
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG170240.2824855007148423Illumina Single End PCR Primer 1 (100% over 46bp)
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG166140.27568221974132934No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT80730.13395826170529382No Hit
CGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTC80630.133792328023013No Hit
CTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATG76810.12745366135988626No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG74940.1243507015012352No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA72490.12028532628535549No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG70220.11651863169758123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)