FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7224590
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2998594.150533109837375No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC217820.30149807809162876No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA203960.2823135984187338No Hit
AGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTATGCCGTCT185550.2568311834996865TruSeq Adapter, Index 8 (96% over 30bp)
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG140610.19462696152999687No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA134320.18592058511278842No Hit
AAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTATGCCGTC124560.1724111679693934TruSeq Adapter, Index 8 (96% over 31bp)
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC108460.15012616632916193No Hit
GAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTATGCCGTCTT85000.11765373536768177TruSeq Adapter, Index 8 (96% over 29bp)
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT81360.11261538717075986No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTATGC78720.10896120056639892TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)