FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004174338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200211
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATGCCGTCT329310.7840320402951186TruSeq Adapter, Index 7 (96% over 28bp)
AAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATGCCGTC248510.5916607522812544TruSeq Adapter, Index 7 (96% over 29bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174660.4158362520359097No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATGC103150.24558290047809506TruSeq Adapter, Index 7 (96% over 33bp)
GAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATGCCGTCTT93710.22310783910617824TruSeq Adapter, Index 7 (96% over 27bp)
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG93360.22227454763582116No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC86230.20529920996826112No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA73190.1742531506155286No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG57140.13604078461772517No Hit
CCACCTCGCCCCGACAGTCCTGCACCTCGAGGTCTCCGCTTTCCTGGAAATCCAGACCCATGG48580.11566085608556331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)