FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9114786
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1189691.3052308633466545No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTAT643510.7060067016384148TruSeq Adapter, Index 1 (97% over 37bp)
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC298360.32733626439501706No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA240030.26334134449234464No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT121520.13332183553184904No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG112060.12294309487902405No Hit
CCATGAACTGGAGCTTTATCACCTTCACAATGGAGTACTCACTGCCTATG95420.1046870436672896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)