FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5782991
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1630452.8193887903335835No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA220290.3809274474056764No Hit
AGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTATGCCGTCT183900.3180015324250029TruSeq Adapter, Index 22 (96% over 31bp)
AAGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTATGCCGTC141950.2454612154852048TruSeq Adapter, Index 22 (96% over 32bp)
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC98020.16949706475420764No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG81190.1403944775290157No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC80150.13859610018414345No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTATGC70430.12178818884552994TruSeq Adapter, Index 22 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)