FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5056064
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC176360.34880887583701475No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA135170.2673423437678004No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG129130.25539629245199424No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122990.24325245882963506No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA98820.19544847533575524No Hit
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACG71420.14125612334021087No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT62500.123613941595676No Hit
CCACCTCGCCCCGACAGTCCTGCACCTCGAGGTCTCCGCTTTCCTGGAAATCCAGACCCATGG54440.10767268768749763No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC51790.10243145656384098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)