FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7208418
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1627112.257235915009368No Hit
AGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTATGCCGTCT199900.2773146618300992TruSeq Adapter, Index 7 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTAT196350.27238986418379174TruSeq Adapter, Index 7 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTATGCCGTC175330.2432295130498814TruSeq Adapter, Index 7 (96% over 31bp)
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA167810.2327972656413654No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC154190.2139026898828564No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA108270.15019939187766304No Hit
CTGCAAGGTCTTCTTCAAAAGAGCCGCTGAAGGTAAAGGGTCTTGCACAT91670.12717076063014102No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG80090.11110620943458052No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTATGC74090.10278260777884966TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)