FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7208418
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1676932.3263495540907866No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG247020.3426826801664387Illumina Single End PCR Primer 1 (100% over 50bp)
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG157590.21861939748777054No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG100410.13929547370865564No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA99090.13746428134439484No Hit
CTGGGACCACCTTCACTGCCTACATTGCCTGTACAAACAAGGCTGCTGAA88110.1222320903144074No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG74030.10269937176229237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)