FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5406512
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1017491.881971222851258No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC143180.2648287842512881No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA111660.20652871944055615No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA91400.1690553909803585No Hit
AGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCCGTCT87740.1622857768557621TruSeq Adapter, Index 1 (96% over 30bp)
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT86180.15940036755675377No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG76470.14144054429177258No Hit
AAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCCGTC71040.13139710038560906TruSeq Adapter, Index 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)