FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5406512
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1023241.892606545587987No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT98800.18274258893719278No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA98630.1824281533084547No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG92440.1709789971796974No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG69350.12827124031168338No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG58380.10798089415134933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)