FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004174352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4478359
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTC705681.5757557623227614TruSeq Adapter, Index 23 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCT528871.1809459670383726TruSeq Adapter, Index 23 (96% over 30bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGC323270.7218492309348133TruSeq Adapter, Index 23 (97% over 35bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN227300.507551985001649No Hit
GAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCTT157000.3505748422580682TruSeq Adapter, Index 23 (96% over 29bp)
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC112120.2503595625093924No Hit
GCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCTTCT100130.22358636277261382TruSeq Adapter, Index 23 (96% over 27bp)
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA76670.17120110290398782No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA69090.15427526020133714No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG59250.13230292613879324No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGT52480.11718578166690075TruSeq Adapter, Index 23 (96% over 32bp)
GTGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGC49650.11086650266314067TruSeq Adapter, Index 23 (96% over 33bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTAT47340.10570836326431177TruSeq Adapter, Index 16 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)