FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7491155
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN965401.2887198302531453No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA223660.29856544151068826No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC183760.24530262689798835No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA139340.1860060297777846No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT104360.13931096072634994No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG93000.1241464099995261No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC78010.10413614455981755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)