FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5514964
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN621761.1274053647494344No Hit
GTGGCAGGAGGAGGCCGTGATGATCACAGATGCAGAAGGCCAGGCATGGA403610.7318452124075515No Hit
CAGGAAAGCATCTTCACCCACAGCAGTGTGGTCATTCTTGACGAGGTCCA295920.536576485358744No Hit
CATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCT266370.4829949932583422No Hit
GACCCCCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGC235100.426294713800489No Hit
CTGAAAATGCAGAATACCTAAGGGTCGCGCCACAAAGCAGTGAATTTATT228990.4152157656876817No Hit
CCCTCCCGAGGCTCCTGCTCCCTGTCATAAGTCTCCTTGTTGAGGACATT225280.40848861388759744No Hit
CTCCTTCAGTCCGGTTTTATTTGCATCATAGTTAGATAAGACTGCTAAGG209780.3803832627012615No Hit
TGCACCAAGCGACGGTCCTCCAAGTAGTTCATGCCCTGAAACAGAGAAGA209330.37956730089262597No Hit
GGGGTGACCGTTTGGGAGTTGATGACCTTTGGATCCAAGCCATATGACGG194390.3524773688459254No Hit
TCCAGAGGAGGAGTATGTGTGAAGGAGTCACTGAAACAAACAACAGGGTG194280.3522779115149256No Hit
CAGCACACTGGTTGTGGCAGCAGTCACTGGGGGACTTGCCACGGCAGCGC190590.34558702468411395No Hit
CGCTCCACTGTGTTGAGGGCAATGAGGACATAACCAGCCACCTCCTGGAT183580.3328761529540356No Hit
CCGCGCCCAGCAGAGACCCACACTACCAGGACCCCCACAGCACTGCAGTG181170.3285062241566763No Hit
AGCCCACCCAGGACTGGCACTCACCTCCCTCTCCTGCAGCAGCCTCCGCA164980.2991497315304325No Hit
CAGTGGGCAACCCCGAGTATCTCAACACTGTCCAGCCCACCTGTGTCAAC161570.2929665542694386No Hit
GGCTCCCGCTCTGGTGGGACACGCTGCCATCATTACTTTGATTACAGGAT159830.28981150194271443No Hit
TGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGATGCCGACGAGT159330.2889048777108971No Hit
AGTCAGAAATGCAGGAAAGCATCTTCACCCACAGCAGTGTGGTCATTCTT155730.2823771832418126No Hit
AGGCATGGAGGTCCGTCCTGTTTTCAGGCCAAGCCTGAATCAGCAAAAAC151030.2738549154627301No Hit
CGTCCCGGAGGGTCGCATCGCTGCTCCCCGAAGAGCTCGCTCCGGCTCTC150250.2724405816610952No Hit
GTGACCGTTTGGGAGTTGATGACCTTTGGATCCAAGCCATATGACGGAAT143710.26058193670892504No Hit
CAGCGATACAGCTCAGACCCCACAGGCGCCTTGACTGAGGACAGCATAGA138890.25184207911420636No Hit
CAGCCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACCGTGCCGAACG136300.2471457655933928No Hit
CTACTAGTTGGGGTGAGAGGCACTGGCAAAGGGAGTGGAAGGAAGGAAAT131330.23813392072912898No Hit
CTGGGGGGGACAAGAACACAGAGACAAGGGTCACCTCAGCCCTCACCATG129530.23487007349458672No Hit
GCCACCCATATGTACCATCGATGTCTACATGATCATGGTCAAGTGTGAGT129060.2340178467166785No Hit
TGTTTTTTCTCATTCCTTCCCCAGGCTGGATGATAGACGCAGATAGTCGC128940.23380025690104234No Hit
GCAGCTGGGCTCCCGCTCTGGTGGGACACGCTGCCATCATTACTTTGATT128420.23285736769995233No Hit
CCTTGGCACTCAACACGCCCCTGCATGTTTCAGTGCACTGACATCTTGTA126130.22870502871822915No Hit
CACCAATACCTATTCCGTTACACACTGCAGAGATAAGAGAGACGTGTATT124930.22652913056186766No Hit
CTGTGGGGACCAAAACACCTTAAGTAACTGACTCAAATACAAACCTCGGC120930.21927613670732937No Hit
TGTTCGGCTTCTGTGAAGGCCCTTCCCATGAAATGGACGTGGATAGCAGC120200.2179524653288761No Hit
CACGTGGCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGAC116930.21202314285279103No Hit
GGCAAACACACAGGCACCTGCTGGCAATAGACCCCTGCTCCTATAGCCAA109310.19820618955989558No Hit
TGCCAAAAATAACGCAATCAAACAACTTCCCGTCTCCCCACAGAAAACCC108480.19670119333507888No Hit
CAGGTTTTCCAAAGGAATTCGCTCCACTGTGTTGAGGGCAATGAGGACAT108130.1960665563728068No Hit
AACAATACAGCTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATG105870.19196861484499264No Hit
CTCCCAAAATAGCCCACCCTGGATTAACTGCATGCGGTGAGATTTGCAAG103850.1883058529484508No Hit
CACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGT103700.18803386567890562No Hit
CGGGCGGGAGGGAGAAAGCGTGTTTACACCGCAGTGAATGACCCAAGCCT103540.18774374592472406No Hit
GCAGGAGACAGAGCGGGACAAGGATGCCTGACCAGTTAGAGGGCCCACAG96880.1756675111569178No Hit
TGTGTTGAGGGCAATGAGGACATAACCAGCCACCTCCTGGATGGTCTAAG96360.1747246219558278No Hit
AGCCTAATTTCCAACTAAAAAGGCAAATGTAATCTACCAGGCTTTGGCTG95860.17381799772401052No Hit
AAACACCTTCACAATATACCCTCCATGAGGCACACCACCTGCATTCAGGA90960.1649330802522011No Hit
GAAGGGTGAGGTTGCTGACAGATTCCATGGCCTTGGCACTCAACACGCCC90620.16431657577456535No Hit
CCGTCAATGTAGTGGGCACACTGGATACAGTTGTCTGGTCCCTGCACCAA88610.16067194636265986No Hit
GCCCACCCAGGACTGGCACTCACCTCCCTCTCCTGCAGCAGCCTCCGCAG88500.16047248903166006No Hit
TGCAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGG87710.15904002274538873No Hit
CCCGCTGGCTCTGTGCAGAATCCTGTCTATCACAATCAGCCTCTGAACCC86740.1572811717356632No Hit
TGGTGGGTATAGATTCTGTGTAAAATTGATTCCAATGCCATCCACTTGAT85970.15588497041866456No Hit
CCAGCCCAAAATCTGTGATCTTGACATGCTGCGGTGTTTTCACCAGTACG85640.15528659842566514No Hit
AAAAGGCAGATTTTGTTTTTCTTTATACAAGCAACTGAACCTGTGACTCA84600.1534008200234852No Hit
TCACATCGAGGATTTCCTTGTTGGCTTTCGGAGATGTTGCTTCTCTTAAT83510.15142437919812352No Hit
CGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTC80960.14680059561585532No Hit
CTTTGTGGACATTGAAGGGTGCAGCCTAGAAACATTGCCATTTCCTCGGA80830.14656487331558282No Hit
CAGAATACCTAAGGGTCGCGCCACAAAGCAGTGAATTTATTGGAGCATGA80680.14629288604603766No Hit
CCAAGCCTGAATCAGCAAAAACCCTGTAAGGCAGATGAAAGACTTCAGAC80630.14620222362285593No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA79280.14375433819694924No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG77850.1411613928939518No Hit
AGCTCACCCAGAATGTCTGGAGAGCATCCTCCCCTGCATGTGTTAAACAA76110.13800634056722763No Hit
GCAGCGCCCGGAGCACTGCTGGGCACAGATGATTTTGGTCACTGAAAAAG74870.13575791247232077No Hit
CTCAGACCCCACAGGCGCCTTGACTGAGGACAGCATAGACGACACCTTCC72770.13195009069868815No Hit
AAATGGGAATAGCCCTTCAATATTCTAAATACAAATAAAGGACCCATTAG71990.13053575689705318No Hit
CCATATGTACCATCGATGTCTACATGATCATGGTCAAGTGTGAGTGACTG71330.12933901291105437No Hit
AGCATTCAACTGGGAGAGACTTTGTTATGCATCATTGTCAGGTAAGGAAA71190.12908515812614552No Hit
CTGTGGCTGCTGGATGAGTGGTGAGGGCTGAGCAGCAGGTGTTTCTCCTG70570.12796094407869207No Hit
CTTGTTACTCGTGCCTTGGCAAACTGGAAGAAAAAGACAGATATCACATG69230.12553119113742175No Hit
GCTTGCATTGATAGAAATGGGGTATGTATGAACACCTTATAAGCCAGAAT66680.12090740755515358No Hit
GCTGTTTTCACCTCTGTTGCTTATAATTTTGGTTTTCTGACCGGAGGTCCCAAAC65840.11938427884570053No Hit
GCTGCTGGAGGGGCATCTTACCAGGCAGTCGCTCTCCCGGGGGCCTGTGC65740.11920295399933709No Hit
AGAGAACTCATGAGATCAAGTCTAGACTTTGAGACCTGCTAAATAATTAG65280.11836885970606519No Hit
GGAGCTGGAGGCAGAGATAGTGTGTATGCGACACTTACAGCTGCCCAGGT64920.11771609025915672No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC63810.11570338446452233No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT63510.11515940992543197No Hit
CCCAGAGCCATAGAAACTTGATCAGGACAGAGGACAGTCAGAAATGCAGG62600.1135093538235245No Hit
ACCCCAGCAGCTCCCATTGGGACAGCTTGGATCACACTTTTGGCCTGAAA61820.11209502002188954No Hit
CATGTGTTAAACAATACAGCTAGTGGGAAGGCAGCCTGGTCCCTGGTGTC61720.11191369517552607No Hit
CCCCACAGGCTCGGACGCACGAGCCGTGATCTGTCACCACATAATTACCT60840.11031803652752764No Hit
ATGAGAAGAGGAAGATGTGTTCCTTTGGAGGTGGCATGAGAGCCCAGCCT60070.10892183521052903No Hit
CCCTTGTTCCTGTCGGGGTGCGAGGGCTCCTGAGCCCACCCAGGACTGGC59900.10861358297171114No Hit
AAACAGTAACCAGTACTAGCTGGCCAAGACAGAAAAGTCTACCACAAAGA59660.10817840334043885No Hit
CATCCACCCCAACCCAGCTGAAACTCTGACGGCAGAAGAGAAGGGATCCA59580.10803334346334809No Hit
GTAAATACATGCTTTTCTAGTGGTCAGATATTTTGCTTACTACTTTTTGT59370.10765256128598483No Hit
CTCCAGAGGGGGAAACAGTGGCAGATTTGCAGACACAGTGAAGGGCGTAA59300.1075256338935304No Hit
CGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTTT57040.10342769236571626No Hit
GGTGGAGATGGCCACCACCTGCCCGCTCCTCGGTAGCAAGCCAGCCCCTC55920.10139685408644553No Hit
AGTGCCAGCATGGGATCAACAGCCAGGCTTCCTGGCCTCCCTCCTATGCA55910.10137872160180918No Hit
GTAGACTGAGGCTTCCAGCATTGAAGCAAATTGCCCAAGACTACAGAAAT55610.10083474706271882No Hit
GGCCCCCACCATCCCAGTGGCGATGGACGGGATCTTAGGCCTGGAATGAG55500.10063528973171901No Hit
GGCTCTTACCCGTCCTGTGCAGGTGATGTTCATGGCCTGAGGCAGGCACT55240.10016384513117402No Hit
CTGCCAGACATGAGAAAAGGTGGGCCTGAGGTTCAGAGCCATGGACCCCC55180.10005505022335594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)