FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6558515
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN370890.5655091129623093No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC234610.3577181724826428No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA223790.34122053544133085No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA183670.28004815114397086No Hit
AGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATGCCGTCT157510.24016107304778597TruSeq Adapter, Index 7 (96% over 28bp)
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG147170.2243953089990646No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG132580.20214941949511436No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT114080.17394181457235364No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC74990.11433990773826089No Hit
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACG72160.1100249065527791No Hit
CCATGAACTGGAGCTTTATCACCTTCACAATGGAGTACTCACTGCCTATG68620.1046273432324238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)