FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5851468
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN922931.5772623211816248No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC174990.29905316067694465No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA124320.2124595058880951No Hit
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACG101280.17308477120613153No Hit
AGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTATGCCGTCT84140.14379297639498329TruSeq Adapter, Index 10 (96% over 30bp)
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA74560.1274210164013543No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG70490.1204654968633512No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT62190.10628102212983134No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG61110.1044353314416143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)