FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6796913
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN802111.1801092643086648No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA170990.2515700877736702No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC132160.19444121176775397No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA127170.18709964361762466No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG115130.16938571966420637No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT96480.1419467926101158No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG95430.1404019736606898No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC79950.11762692857772346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)