FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5474308
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN909241.6609222572058422No Hit
AAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTC360200.6579827075860547TruSeq Adapter, Index 23 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCT267490.4886279690510654TruSeq Adapter, Index 23 (96% over 30bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGC233180.42595338077433714TruSeq Adapter, Index 23 (97% over 35bp)
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA171650.31355561287380984No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG130110.23767387585791666No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA96270.17585784358497913No Hit
GAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCTT90130.16464181408864828TruSeq Adapter, Index 23 (96% over 29bp)
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC84630.15459488212939423No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC81060.14807350993038754No Hit
TCAGCCCAGGTAAGGTGGCCATAGGCCCTGATGACCCAAAACCCCAGGCT78890.14410953859373643No Hit
CACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCTTCTG64830.11842592707607974RNA PCR Primer, Index 46 (96% over 27bp)
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT62450.1140783456100753No Hit
TCAGTGGATCCTTATCACCTTCATAGAAAGGTACTCACCTCCCACGTCAA59120.10799538498747238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)