FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5474308
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN930551.6998495517606973No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG148980.2721439860526664No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG97210.17757495559256076No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG86400.15782816750537235No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT73800.13481155974417225No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG63640.11625213634307752No Hit
CTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATG61540.11241603504954416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)