FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8153649
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN512680.6287736938394086No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG236040.28949001851808925No Hit
CGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTC146290.17941660230897846No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT144740.17751561294826404No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG141900.17403250986153562No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG127240.15605282984342347No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA110120.13505609574314517No Hit
CTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATG102220.12536718222724574No Hit
CTTAGTGGAAAAAAACACCATTGATACAACTGTCATTGTGAAAGTATGGA83290.10215058313155251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)