FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6032947
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN977821.6207999175195802No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA152620.25297752491444064No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG142170.2356559737720222No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC97270.16123131862421466No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT72240.11974247411754156No Hit
CCATGAACTGGAGCTTTATCACCTTCACAATGGAGTACTCACTGCCTATG66430.11011202319529743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)