FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9734352
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1422691.461514849678746No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT186430.19151762747022094No Hit
CACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCCGTCTTCTG168220.17281068118350354TruSeq Adapter, Index 1 (96% over 26bp)
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC122100.1254320780674461No Hit
AGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCCGTCT114400.1175219470181477TruSeq Adapter, Index 1 (96% over 30bp)
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA105970.10886189445378594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)