FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12688762
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4776543.7643861552450897No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC165960.1307929016242877No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC161160.12701002666769223No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG158320.12477182565170661No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA154360.12165095381251535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)