FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8268770
Sequences flagged as poor quality0
Sequence length32-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2141262.589574991201835No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA168960.20433510667245552No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC166940.2018921798526262No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG161830.19571230062028572No Hit
AAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTATGCCGTC147990.1789746237953166TruSeq Adapter, Index 13 (96% over 32bp)
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC142390.1722021534037106No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC104690.12660891523164874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)