FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8377681
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4924765.878428648691685No Hit
AAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTC179480.21423589654464048TruSeq Adapter, Index 18 (96% over 31bp)
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA177330.21166955390161074No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGC157690.18822631226946934TruSeq Adapter, Index 18 (97% over 35bp)
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG147060.17553783678323392No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC122280.14595924576264005No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC110440.13182645650986233No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC86020.1026775786760083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)