FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9256469
Sequences flagged as poor quality0
Sequence length32-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3648273.9413193086910354No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG150800.16291309353491057No Hit
AAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTATGCCGTC145120.15677684438850278TruSeq Adapter, Index 23 (96% over 31bp)
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA132450.14308911962001925No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC118200.12769448047630255No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC115090.12433466800353353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)