FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14267932
Sequences flagged as poor quality0
Sequence length32-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9498176.657005374009352No Hit
AAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTATGCCGTC206330.14461100599582338TruSeq Adapter, Index 2 (96% over 32bp)
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA197780.1386185468223426No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG192730.1350791411116902No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTATGC176900.12398433073552634TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)