FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10060603
Sequences flagged as poor quality0
Sequence length32-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5362185.329879332282568No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT387160.38482782791448983No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC305180.30334165854670936No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC248610.24711242457335808No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC221870.22053350082495055No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA204360.2031289774579118No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG185350.1842334897818749No Hit
GAGAGCAGGCCGGCCCCGGGCTCCACCGCGCCCGCAGCCCACGGTGTCACCTCGGCCCCG124310.12356118216770903No Hit
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCCCCGCCCCC102690.1020714165940153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)