FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8303271
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5379096.478278259254696No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA149120.17959187409395647No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG130450.15710675949273484No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC126450.15228938089579397No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC125160.15073577629828053No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC92630.1115584448586587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)