FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10486545
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3529443.3656843126120184No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA189720.18091754719976885No Hit
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTC174030.16595551728429145TruSeq Adapter, Index 22 (96% over 32bp)
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT164330.15670556889804985No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG158870.15149889691981486No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC113000.10775713068508265No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC112630.10740429760230848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)