FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10130451
Sequences flagged as poor quality0
Sequence length32-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3643933.597006688053671No Hit
AGATGGGGGCAAGGTTAGGTGAAGGACCAAGGAGCAGAGGAGGCTGGGTG257760.25444079439306305No Hit
TGATGGCCACTGGAATTTTCACATTCTCCCCATCAGGGATCCAGATGCCC213530.2107803492657928No Hit
TCAGTGTGCTATGGTCTGGGCATGGAGCACTTGCGAGAGGTGAGGGCAGT210670.2079571778196252No Hit
CTGGAGGCAGGGCCTGGCTGGGTTGCCCACGGGCCTCACCTGCAGGAAGG182540.18018941111308864No Hit
CCTGTCCCCACTCCTTTAATCTCACCCTCTGCCTGCAGGTTACCTATACA162550.1604568246764137No Hit
CCCTGTCTTACCACACTCGTCCTCTGGCCGGTTGGCAGTGTGGAGCAGAG154860.152865849704026No Hit
TTAGGTGTGACTGTGTGGGAGCTGATGACTTTTGGGGCCAAACCTTACGA154460.15247100055071586No Hit
TCAGCCTGTCTGGGTCCCTCCCTTGCTGTGCAGTTGGCCTCGTGGCCTCT140740.13892767459217759No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA136720.13495944060141055No Hit
AAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTATGCCGTC136140.13438690932911082TruSeq Adapter, Index 7 (96% over 29bp)
CTCTTTGAGGACAACTATGCCCTGGCCGTGCTAGACAATGGAGACCCGCT135320.133577468564825No Hit
CCCCAAGCACTCTGACTGCCTGGTATGTGCCTCTGCTTTGTGCCCAATGT133720.1319980719515844No Hit
CTTTCTCTCCTCCCTGGGGAATCTGGGGGTTGTTTAAAAGGCCTGCTCCT131600.12990537143904057No Hit
CCGGACAGCCTGCCTGACCTCAGCGTCTTCCAGAACCTGCAAGTAATCCG131010.1293229689379081No Hit
TGAGGACACATCAGGGCTTCTGCGGACTTGGCCTTCTGGTTCACACTGGC128050.12640108520341295No Hit
AATTGATAGTCAAGGAGGGGGATGTGTTGTGTTTACCTTGTGGCTTCCAG123500.12190967608450996No Hit
CGAAGGGCCAGGGTATGTGGCTACATGTTCCTGATCTCCTTAGACAACTA120550.11899766357884758No Hit
CCCTCATCCACCATAACACCCACCTCTGCTTCGTGCACACGGTGCCCTGG120120.11857320073903915No Hit
TATTTGATGGTGACCTGGGAATGGGGGCAGCCAAGGGGCTGCAAAGCCTC118830.11729981221961393No Hit
GGAGCTGAGGAAGGTGAAGGTGCTTGGATCTGGCGCTTTTGGCACAGTCT113740.11227535674374221No Hit
CTCCCGCAGGCCTCCTGGGGAGGCCCCTGTGACAGGGGTGGTATTGTTCA112980.11152514335245292No Hit
AGAGGTGAGGGCAGTTACCAGTGCCAATATCCAGGAGTTTGCTGGCTGCA112850.11139681737762713No Hit
CCTCCCTGAGACTCACGGCTCTGACAATCCTCAGAACTCTCTCCCCAGCA108820.10741871215802731No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT105020.10366764520158085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)