FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13060582
Sequences flagged as poor quality0
Sequence length32-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2595281.987108997133512No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT360950.2763659383632368No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA202580.15510794235662698No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC193200.14792602657370094No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC181690.1391132493176797No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTACAAGATCTCGTATGCCGTCT180550.13824039388137527TruSeq Adapter, Index 14 (96% over 31bp)
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG178720.13683923120730762No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC151250.11580647784302414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)