FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11931485
Sequences flagged as poor quality0
Sequence length32-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2793842.3415693855375084No Hit
AGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGG306460.2568498388926441No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248250.2080629527674049No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG163260.1368312494211743No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA138580.11614648134746011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)