FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14932878
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5807783.889256980469538No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA252960.16939802227005404No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG213400.14290614307570182No Hit
AGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCT156770.10498311176184523TruSeq Adapter, Index 18 (96% over 30bp)
AAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTC155990.1044607744066482TruSeq Adapter, Index 18 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)