FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14735588
Sequences flagged as poor quality0
Sequence length32-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5927654.022676258321011No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA321830.21840322897192835No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT278150.18876070639325693No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG253690.17216143665254485No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC221780.15050637952146873No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC197900.13430071470510713No Hit
AAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTC189470.12857987071842672TruSeq Adapter, Index 5 (96% over 32bp)
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC175880.11935730016338676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)